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Korean J Parasitol. 2009 Dec;47(4):413-415.
Published online 2009 December 02.  doi: 10.3347/kjp.2009.47.4.413.
Copyright © 2009 by The Korean Society for Parasitology
Genotype of Toxoplasma gondii from Blood of Stray Cats in Gyeonggi-do, Korea
Hye-Youn Kim,1,2,3 Yun-Ah Kim,1 Ho Sa Lee,2 Ho Gun Rhie,2 Shin-Hyeong Cho,1 Jae-Ran Yu,1,4 and Sang-Eun Lee1
1Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul 122-701, Korea.
2Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
3Department of Biotechnology, The Catholic University of Korea, Bucheon 420-743, Korea.
4Department of Environmental and Tropical Medicine, Konkuk University, School of Medicine, Seoul 143-701, Korea.

Corresponding author (Email: ondalgl@cdc.go.kr )
Received June 05, 2009; Revised September 29, 2009; Accepted October 05, 2009.

Abstract

Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2 gene, amplifying the 5'and 3'ends of the locus. The results revealed that all samples belonged to the type I clonal lineage. Although T. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with T. gondii infection should be seriously concerned in our environment. Adequate and continuous control programs of stray cats are needed to reduce the risk of transmission of T. gondii as a zoonotic infection threatening the public health.

Keywords: Toxoplasma gondii, PCR-RFLP, stray cat, SAG2.
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